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Humane Euthanasia - HEPP

Sample Protocol for Lethal Injection of Sodium Pentobarbital
(Summary)

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Also see: Sample Protocal Full Version

1. Set up designated euthanasia area:

a) Ensure that the designated euthanasia space is fully stocked with necessary equipment and supplies and offers good overhead lighting as well as a sturdy table;

b) Ensure the euthanasia room is quiet and does not allow other staff or people to pass through during euthanasia. Relaxing music at soft volume levels can be played to further enhance the quiet atmosphere;

c) If animals are to be euthanized in their "home" kennel rather than further stressing animals by removing them and taking them to a designated room, ensure that other animals cannot witness euthanasia or carcasses by draping kennels with sheets or blankets.

2. Staff performing euthanasia are trained in a certified course supplemented by on-the-job training, oversight and support:

a) Random audits of procedures and proficiency are conducted by administration, consultants or veterinarian to ensure that humane procedures are being strictly adhered to;

b) Administration remains current on euthanasia procedures and is committed to providing proper facilities, equipment, supplies, training programs and stress management tools for employees participating in euthanasia.

3. Animals are selected for euthanasia according to shelter/department policy which includes Temperament Testing, psychological breakdown, and health, etc

4. Prior to preparing the animal for euthanasia and prior to euthanizing, paperwork is checked by two staff members to ensure the animal being euthanized has been selected for euthanasia and to ensure mistakes are not made resulting in euthanasia of the wrong animal:

a) Staff checks for identification, tattoos (inside lip, flank or ear) or microchips on intake and re-check prior to euthanasia.

b) Staff re-verify all paperwork including cage/kennel card, intake card, animal ID information, paying special attention to holding period times and pending adoptions, re-claims or "special holds" on the animal.

5. Staff perfoms euthanasia in accordance with humane standards taught in certified training course:

a) Animals are euthanized via an intravenous injection whenever possible, with intraperitoneal injections allowed on some animals such as feral cats, birds and small novelty pets. Intracardiac injections are only used on animals sedated to a surgical level, verified through toe-pinch or eye reflex.

b) IV injections are given into the cephalic vein (preferred site for IV injection; located on top of the front paw) whenever possible, with alternate sites allowed as per certified training.

c) Administers anesthetic anytime an animal is in distress or at risk of pain or suffering during the procedure.

6. Staff evaluates animals scheduled for euthanasia and pre-anesthetizes as necessary using a recommended mixture of: Xylazine (1 mg/lb) with Ketamine (5 mg/lb).

Formula One: The suggested mixture by volume is 1:1, such as 0.5 cc of Xylazine (20 mg/cc) + 0.5 cc of ketamine (100 mg/cc). Note: Ensure the produce used is Small Animal Xyalamine or small animal Rompun" when using this dosage. Dosage is: 1 cc of ketamine/xylazine mixture per 10 lbs given IM ­ sedation should occur within five minutes.

Formula Two: Add 2 ml of "large animal Xylazine (100 mg/ml) to a 10 ml vial of ketamine. Label the vial with the date, the amount of xylazine added and your initials. Dosage for dogs, cats, raccoons, opossum and skunks is: 0.6 ml per 10 lbs, IM or SQ.

 

Suggested dosage chart for ketamine/xylazine mixture:

0.6 mls - 10 lbs

0.9 mls -15 lbs

1.2 mls - 20 lbs

1.5 mls ­ 25 lbs

1.8 mls ­ 30 lbs

2.1 mls ­ 35 lbs

2.4 mls ­ 40 lbs

2.7 mls ­ 45 lbs

3.0 mls ­ 50 lbs

 

a) Animals who are unsocial, fearful, fractious, aggressive, dangerous, dehydrated or scheduled for euthanasia using an intracardiac injection, or scheduled to be euthanized without the assistance of a handler are anesthetized to a surgical level (verified by a toe pinch or eye reflex) prior to euthanasia.

7. Handler duties while holding animals for euthanasia:

a) Ensures procedure is performed according to humane standards, accredited training guidelines and department protocol, including controlled substance guidelines;

b) Re-checks paperwork and animal identification;

c) Speaks comfortingly and reassuringly to the animal throughout the procedure;

d) Restrains animal and responds to directives from the injector;

e) Holds the animal so the animal's head is away from the injector;

f) Gently but firmly holds the animal against the body without restricting breathing or inducing distress;

g) Applies gentle pressure to the cephalic vein or other vein with the thumb, slightly rotating the hand and thumb clockwise (laterally) to provide tension on the vein;

h) Releases pressure on the vein when instructed to do so by the injector;

i) During injection of the cephalic vein, the handler keeps the leg extended by maintaining pressure on the back of the leg, just above the elbow;

j) Maintains mild restraint until the animal succumbs to the drug and collapses;

k) Works with injector to verify signs of death using as many recommended methods as necessary to verify death, including: watching for signs of breathing; palpating the animal for a heartbeat, breath or pulse; toe pinch; checking for a heartbeat with the stethoscope; eye reflex; Rigor Mortis (facial muscles first in 1/2 to one hour); and by inserting the needle into the heart to check for movement.

l) Helps with carcass disposition after death has been verified absolutely. Animal is not left alone, bagged or placed in refrigerator/freezer or other disposal vehicle until death is verified.

m) Works with injector to clean table between animals;

n) Works with injector to complete all paperwork and drug logs;

o) Works with other staff to clean and re-stock euthanasia room as necessary;

p) Conveys concerns and staff needs to administration as necessary to ensure equipment and supplies are well stocked and available and that staff is provided with needed support.

8. Injector duties while performing euthanasia:

a) Ensures procedure is performed according to humane standards, accredited training guidelines and department protocol, including controlled substance regulations;

b) Verifies paperwork and animal identification;

c) Calculates drug dose and fills syringe using one needle to draw the drug and a new sharp needle to inject the animal;

d) Selects a new needle of appropriate size for each animal being euthanized, ensuring there are no barbs on the needle;

e) Clips or trims the injection site as necessary to assist in finding the vein, using warm water first, and alcohol second, to help visualize the vein as needed. Cats should not be subjected to the electric clipper.

f) Remains calm, speaking comfortingly and reassuringly to the animal;

g) Is prepared to offer anesthetic at anytime during the procedure as needed to ensure the animal is not stressed, pained, frightened or suffering;

h) Inserts needle low on the leg, with the bevel side up when animal is properly restrained and vein has been visualized;

i) Checks insertion by aspirating blood into the syringe;

j) Instructs handler to release pressure on the vein to allow drug to enter the bloodstream;

k) Injects drug steadily and as rapidly as possible, ensuring needle remains inside of vein and dose does not "bubble" under the skin;

l) Removes needle when dose has been delivered and disposes of needle in an appropriate medical waste container;

m) Works with handler to verify signs of death using as many recommended methods as necessary to verify death, including: watching for signs of breathing; palpating the animal for a heartbeat, breath or pulse; toe pinch; checking for a heartbeat with the stethoscope; eye reflex; Rigor Mortis (facial muscles first in 1/2 to one hour); and by inserting the needle into the heart to check for movement.

n) Helps with carcass disposition after death has been verified absolutely. Animal is not left alone, bagged or placed in refrigerator/freezer or other disposal vehicle until death is verified.

o) Works with handler to clean table between animals;

p) Works with handler to complete all paperwork and drug logs;

q) Works with other staff to clean and re-stock euthanasia room as necessary.

r) Ensures controlled substance logs are fully complete and returns drugs to locked safe according to DEA/NMPB regulations;

s) Conveys concerns and staff needs to administration as necessary to ensure equipment and supplies are well stocked and available and that staff is provided with needed support.